Mouse Alanine Aminotransferase(ALT/GPT)ELISA Kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-E16539m

Sensitivity

0.039 mU/mL

Detection Range

0.156 mU/mL-10 mU/mL

Reactivity

Mouse

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

Alanine Aminotransferase(ALT/GPT), AAT1, ALT1, GPT1, glutamic pyruvate transaminase, glutamic-alanine transaminase 1

Recovery

The recovery of human FBLN5 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	90	85-95
EDTA plasma (n=4)	102	98-106

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human FBLN5 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	97
	Range %	92-102
1:2	Average %	91
	Range %	85-97
1:4	Average %	103
	Range %	98-107
1:8	Average %	87
	Range %	82-94

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Mus musculus

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	Q8QZR5

Applications	ELISA
Research Area	ALT, GPT

Storage	Store at 2-8°C. Please refer to protocol.

Reference	FTO-dependent function of N6-methyladenosine is involved in the hepatoprotective effects of betaine on adolescent mice Chen J. et al,J Physiol Biochem,2015 Sep;71(3):405-13. Is plasma soluble CD36 associated with cardiovascular risk factors in early onset coronary artery disease patients? Krzystolik A et al,Scand J Clin Lab Invest,2015 Sep;75(5):398-406. The Effects of Taoren-Honghua Herb Pair on Pathological Microvessel and Angiogenesis-Associated Signaling Pathway in Mice Model of CCl4-Induced Chronic Liver Disease Xi S.et al,Evidence-Based Complementary and Alternative Medicine,/ A water-soluble nucleolin aptamer-paclitaxel conjugate for tumor-specific targeting in ovarian cancer Li F.et al,Nat Commun.,Nov 9 NMI and IFP35 serve as proinflammatory DAMPs during cellular infection and injury Xiahou Z.et al,Nat Commun.,Oct 16 2,3,5,4'-tetrahydroxy‑stilbene-2-O-β-D-glucoside attenuates methionine and choline‑deficient diet‑induced non‑alcoholic fatty liver disease Mingnuan Han.et al,Experimental and TherapeuticMedicine,June 13 2,3,5,4'tetrahydroxystilbene2OβDglucoside attenuates methionine and cholinedeficient dietinduced nonalcoholic fatty liver disease Han.et al,Experimental and Therapeutic Medicine,August 1

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