Mouse adiponectin,ADP ELISA Kit

Mouse adiponectin,ADP ELISA Kit

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Mfr.No.

CSB-E07272m

Alternative Names

adiponectin, C1Q and collagen domain containing, ACDC, ACRP30, ADIPQTL1, ADPN, APM-1, APM1, GBP28, adiponectin, OTTHUMP00000210047, OTTHUMP00000210048, adipocyte, C1Q and collagen domain containing, adipocyte, C1Q and collagen domain-containing, adipo

Reactivity

Mouse

Detection Range

0.156 ng/mL-10 ng/mL

Sample Type

Tissue homogenates, Cell lysates, Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of goat IL-12/P40 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	85	81-90
EDTA plasma (n=4)	92	87-96

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of goat IL-12/P40 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	95
	Range %	89-102
1:2	Average %	86
	Range %	82-97
1:4	Average %	93
	Range %	85-102
1:8	Average %	95
	Range %	89-105

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
600	1.974	1.942	1.958	1.857
300	1.331	1.362	1.347	1.246
150	0.912	0.924	0.918	0.817
75	0.587	0.612	0.600	0.499
37.5	0.401	0.388	0.395	0.294
18.75	0.295	0.309	0.302	0.201
9.37	0.214	0.225	0.220	0.119
0	0.102	0.100	0.101

Species

Mus musculus

Sensitivity

0.039 ng/mL

Limit Of Detection

450 nm

UniProt ID	Q60994
Antigen	adiponectin, C1Q and collagen domain containing

Applications

ELISA

Research Area

ADP

Protocol

Precision

Storage	Store at 2-8°C. Please refer to protocol.

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.