Matrices listed below were spiked with certain level of FL and the recovery rates were calculated by comparing the measured value to the expected amount of FL in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
87-102
95
EDTA plasma(n=5)
93-100
96
heparin plasma(n=5)
89-101
95
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of FL and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
87-104%
89-100%
89-103%
EDTA plasma(n=5)
82-96%
84-96%
84-97%
heparin plasma(n=5)
80-100%
81-96%
82-96%
Sensitivity
< 18.75pg/ml
Notes
For Research Use Only
Applications
ELISA
Applications Notes
Adenovirus ELISA Kit allows for the in vitro quantitative determination of FL concentrations in serum, plasma, tissue homogenates and other biological fluids.