Mouse Acrosin-binding Protein

Mfr.No.

E1811Mo

Sensitivity

0.026ng/mL

Detection Range

0.05-20ng/mL

Reactivity

Mouse

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

ACRBP;Acrosin-binding protein;Acrosin-binding protein, 32 kDa form, mature form;Acrosin-binding protein, 60 kDa form;Acrosin-binding protein, mature form;ACRPB-C;OY-TES;OY-TES-1;Proacrosin-binding protein sp32;sp;sp32;Sp32)

Molecular Weight

61141

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E1811Mo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	6.12	0.29	4.78
Intra-Assay	2	18	8.56	0.3	3.55
Intra-Assay	3	18	2.14	0.1	4.78

Background

Acrosomal protein that maintains proacrosin (pro-ACR) as an enzymatically inactive zymogen in the acrosome. Involved also in the acrosome formation. Maintains pro-ACR as an enzymatically inactive zymogen in the acrosome until acrosomal exocytosis (PubMed:27303034). Partially contributes also to the assembly of acrosomal proteins to form an acrosomal granule (PubMed:27303034). Rodent specific isoform that participates in the formation of the acrosomal granule into the center of the acrosomal vesicle during early spermiogenesis (PubMed:27303034). In the fertilization process promotes ACR release from the acrosome during acrosomal exocytosis (PubMed:27303034).

Protocol

These standard curves of E1811Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
12ng/mL	1.836	1.813	1.765
	1.789
6ng/mL	1.084	1.021	0.974
	0.959
3ng/mL	0.616	0.62	0.573
	0.625
1.5ng/mL	0.221	0.257	0.21
	0.293
0.75ng/mL	0.14	0.114	0.066
	0.087
0ng/mL	0.035	0.047	0
	0.06

Gene ID	54137
UniProt ID	Q3V140

Research Area	Tags & Cell Markers; Cancer

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12109623	E1811Mo	48T	$320		Add to Cart
BHE12109623	E1811Mo	96T	$458		Add to Cart

Mouse Acrosin-binding Protein - ACRBP ELISA Kit

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