Mouse Acetylcholinesterase(AChE)ELISA Kit

Mfr.No.

CSB-E17521m

Sensitivity

0.078 mU/mL

Detection Range

0.312 mU/mL-20 mU/mL

Reactivity

Mouse

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

Acetylcholinesterase(AChE), ARACHE, N-ACHE, YT, OTTHUMP00000211347, OTTHUMP00000211349, OTTHUMP00000211356, acetylcholinesterase, apoptosis-related acetylcholinesterase

Recovery

The recovery of human VEGFR-3/Flt-44 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	86-100
EDTA plasma (n=4)	93	87-98

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human VEGFR-3/Flt-44 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	98
	Range %	91-105
1:2	Average %	90
	Range %	85-96
1:4	Average %	95
	Range %	85-105
1:8	Average %	92
	Range %	83-98

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
10	2.549	2.516	2.533	2.420
5	1.517	1.557	1.537	1.424
2.5	0.796	0.758	0.777	0.664
1.25	0.472	0.489	0.481	0.368
0.625	0.302	0.315	0.309	0.196
0.312	0.259	0.246	0.253	0.140
0.156	0.164	0.171	0.168	0.055
0	0.111	0.115	0.113	?

Species

Mus musculus

Limit Of Detection

450 nm

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Protocol

UniProt ID	P21836

Applications	ELISA
Research Area	AChE

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10505041	CSB-E17521m	96T	$790	Add to Cart
BHE10505041	CSB-E17521m	5x96T	$3792	Add to Cart
BHE10505041	CSB-E17521m	10x96T	$7505	Add to Cart

Mouse Acetylcholinesterase(AChE)ELISA Kit

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