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Home / ELISA Kit

Mouse Acetylcholine (ACH) ELISA Kit

SKU : BHE13705698

Acetylcholine (Ach) is produced by the synthetic enzyme choline acetyltransferase which uses acetyl coenzyme A and choline as substrates for the formation of acetylcholine.Dietary choline and phosphatidylcholine serve as the sources of free choline for acetylcholine synthesis. Upon release, acetylcholine is metabolized into choline and acetate by acetylcholinesterase, and other nonspecific esterases. Acetylcholine release can be excitatory or inhibitory depending on the type of tissue and the nature of the receptor with which it interacts. Cholinergic receptors can be divided into two types, muscarinic and nicotinic, based on the pharmacological action of various agonists and antagonists. Muscarinic receptors originally were distinguished from nicotinic receptors by the selectivity of the agonists muscarine and nicotine respectively.

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Mfr.No.	KTE70539
Reactivity	Mouse
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Mouse Acetylcholine (ACH) ELISA Kit employs a two-site sandwich ELISA to quantitate ACH.
Alternative Names	ACH
Detection Method	Colorimetric
Kit Components	Mouse Acetylcholine microplate Mouse Acetylcholine standard Mouse Acetylcholine detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Mouse Acetylcholine (ACH) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse ACH. No significant cross-reactivity or interference between Mouse ACH and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	Acetylcholine (Ach) is produced by the synthetic enzyme choline acetyltransferase which uses acetyl coenzyme A and choline as substrates for the formation of acetylcholine.Dietary choline and phosphatidylcholine serve as the sources of free choline for acetylcholine synthesis. Upon release, acetylcholine is metabolized into choline and acetate by acetylcholinesterase, and other nonspecific esterases. Acetylcholine release can be excitatory or inhibitory depending on the type of tissue and the nature of the receptor with which it interacts. Cholinergic receptors can be divided into two types, muscarinic and nicotinic, based on the pharmacological action of various agonists and antagonists. Muscarinic receptors originally were distinguished from nicotinic receptors by the selectivity of the agonists muscarine and nicotine respectively.
Notes	This Mouse Acetylcholine (ACH) ELISA Kit employs a two-site sandwich ELISA to quantitate ACH in samples. An antibody specific for ACH has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyACH present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ACH is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ACH bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Applications	ELISA
Applications Notes	This Mouse Acetylcholine (ACH) ELISA Kit employs a two-site sandwich ELISA to quantitate ACH in samples. An antibody specific for ACH has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyACH present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ACH is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ACH bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13705698	KTE70539	48 T	$359		Add to Cart
BHE13705698	KTE70539	96 T	$619		Add to Cart
BHE13705698	KTE70539	96 Tx5	$2809		Add to Cart
BHE13705698	KTE70539	96 Tx50	$26209		Add to Cart

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