Mouse A disintegrin and metalloproteinase with thrombospondin motifs 5(ADAMTS5) ELISA kit

Mouse A disintegrin and metalloproteinase with thrombospondin motifs 5(ADAMTS5) ELISA kit

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Mfr.No.

CSB-EL001312MO

Sensitivity

0.156 ng/mL

Detection Range

0.625 ng/mL-40 ng/mL

Reactivity

Mouse

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

ADAM metallopeptidase with thrombospondin type 1 motif, 5, ADAMTS11, ADMP-2, FLJ36738, a disintegrin-like and metalloprotease (reprolysin type) with thrombospondin type 1 motif, 5 (aggrecanase-2), aggrecanase-2

Recovery

The recovery of human PGAM2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	89-98
EDTA plasma (n=4)	98	92-104

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human PGAM2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	88
	Range %	82-94
1:2	Average %	102
	Range %	98-105
1:4	Average %	95
	Range %	89-99
1:8	Average %	98
	Range %	91-102

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
1000	2.526	2.417	2.472	2.327
500	1.608	1.505	1.557	1.412
250	0.884	0.855	0.870	0.725
125	0.486	0.494	0.490	0.345
62.5	0.329	0.306	0.318	0.173
31.2	0.265	0.257	0.261	0.116
15.6	0.197	0.189	0.193	0.048
0	0.145	0.144	0.145

Species

Mus musculus

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	Q9R001

Applications	ELISA
Research Area	ADAMTS5

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10505080	CSB-EL001312MO	96T	$695	Add to Cart
BHE10505080	CSB-EL001312MO	5x96T	$3336	Add to Cart
BHE10505080	CSB-EL001312MO	10x96T	$6602.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.