Mouse A Disintegrin And Metalloprotease 8,ADAM8 ELISA Kit

Mouse A Disintegrin And Metalloprotease 8,ADAM8 ELISA Kit

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Mfr.No.

CSB-E08817m

Sensitivity

15.6 pg/mL

Detection Range

62.5 pg/mL-4000 pg/mL

Reactivity

Mouse

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

ADAM metallopeptidase domain 8, CD156, MGC134985, MS2, a disintegrin and metalloproteinase domain 8, cell surface antigen MS2, disintegrin and metalloproteinase domain-containing protein 8, human leukocyte differentiation antigen

Recovery

The recovery of human PRDX6 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	100	95-105
EDTA plasma (n=4)	97	93-102

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human PRDX6 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	84
	Range %	80-87
1:2	Average %	92
	Range %	87-96
1:4	Average %	91
	Range %	85-97
1:8	Average %	97
	Range %	91-103

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
800	2.144	2.302	2.223	2.098
400	1.718	1.768	1.743	1.618
200	1.303	1.321	1.312	1.187
100	0.847	0.868	0.858	0.733
50	0.535	0.561	0.548	0.423
25	0.352	0.342	0.347	0.222
12.5	0.286	0.289	0.288	0.163
0	0.124	0.126	0.125

Species

Mus musculus

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	Q05910

Applications	ELISA
Research Area	ADAM8

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
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BHE10505068	CSB-E08817m	10x96T	$6602.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.