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Home / ELISA Kit

Mouse 5-nucleotidase - 5-NT ELISA Kit

SKU : BHE12108007

Hydrolyzes extracellular nucleotides into membrane permeable nucleosides.

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Bioassay Technology Laboratory

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+1 866.986.9598

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Mfr.No.

E0083Mo

Sensitivity

0.033mU/mL

Detection Range

0.06-18mU/mL

Reactivity

Mouse

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

5'-NT;5-NT;5'-nucleotidase;AI447961;CD antigen CD73;CD73;Ecto-5'-nucleotidase;eNT;NT;Nt5;NT5E

Molecular Weight

63864

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E0083Mo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	3.18	0.17	5.21
Intra-Assay	2	18	7.47	0.26	3.5
Intra-Assay	3	18	0.99	0.07	7.23

Background

Hydrolyzes extracellular nucleotides into membrane permeable nucleosides.

Protocol

These standard curves of E0083Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
9.6mU/mL	2.264	2.282	2.232
	2.3
4.8mU/mL	1.335	1.233	1.183
	1.131
2.4mU/mL	0.735	0.752	0.702
	0.769
1.2mU/mL	0.5	0.513	0.463
	0.527
0.6mU/mL	0.209	0.216	0.166
	0.222
0mU/mL	0.06	0.05	0
	0.04

Gene ID	23959
UniProt ID	Q61503

Research Area	Cardiovascular

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12108007	E0083Mo	48T	$320		Add to Cart
BHE12108007	E0083Mo	96T	$458		Add to Cart

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