Mouse 5-Hydroxytryptamine,5HT ELISA Kit

Mfr.No.

CSB-E08365m

Alternative Names

5-Hydroxytryptamine,5HT

Reactivity

Mouse

Detection Range

250 pg/mL-4000 pg/mL

Sample Type

Tissue homogenates, Serum, Plasma

Assay Type

Competitive

Assay Duration

1-5h

Recovery

The recovery of rabbit BMP2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	93	82-100
EDTA plasma (n=4)	97	88-105

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rabbit BMP2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:2	Average %	87
	Range %	80-94
1:4	Average %	90
	Range %	85-99
1:8	Average %	95
	Range %	85-103
1:10	Average %	95
	Range %	84-102

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
100	2.136	2.177	2.157	2.043
50	1.612	1.628	1.620	1.506
25	1.221	1.239	1.230	1.116
12.5	0.852	0.865	0.859	0.745
6.25	0.530	0.516	0.523	0.409
3.12	0.351	0.365	0.358	0.244
1.56	0.239	0.246	0.243	0.129
0	0.112	0.115	0.114	?

Species

Mus musculus

Sensitivity

125 pg/mL

Limit Of Detection

450 nm

Antigen	5-Hydroxytryptamine,5HT

Applications

ELISA

Research Area

5HT

Protocol

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Storage	Store at 2-8°C. Please refer to protocol.

Reference	Physiological and behavioral responses in offspring mice following maternal exposure to sulfamonomethoxine during pregnancy. Zhang Q.et al,Neurosci Lett.,624:8-16 Modulation of TGF-B/Smad and ERK signaling pathways mediates the anti-fibrotic effect of mirtazapine in mice El-Tanbouly DM.et al,Toxicol Appl Pharmacol,Jun 13

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SKU	Supplier No.	Size	Your price
BHE10505034	CSB-E08365m	96T	$835	Add to Cart
BHE10505034	CSB-E08365m	5x96T	$4008	Add to Cart
BHE10505034	CSB-E08365m	10x96T	$7932.50	Add to Cart

Mouse 5-Hydroxytryptamine,5HT ELISA Kit

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