Matrices listed below were spiked with certain level of 25 Hydroxy Vitamin D and the recovery rates were calculated by comparing the measured value to the expected amount of 25 Hydroxy Vitamin D in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
89-105
98
EDTA plasma(n=5)
88-105
95
heparin plasma(n=5)
90-100
96
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of 25 Hydroxy Vitamin D and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
88-100%
87-105%
90-105%
EDTA plasma(n=5)
88-98%
82-101%
82-100%
heparin plasma(n=5)
83-99%
81-99%
86-90%
Sensitivity
< 1.875ng/ml
Notes
For Research Use Only
Applications
ELISA
Applications Notes
25 Hydroxy Vitamin D ELISA Kit allows for the in vitro quantitative determination of 25 Hydroxy Vitamin D concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Mouse 25 Hydroxy Vitamin D (25 Hydroxy Vitamin D) ELISA Kit