Other liquid samples, Serum, Cell culture supernatants, Plasma, Cell lysate
Assay Type
Sandwich
Recovery
Matrices listed below were spiked with certain level of 15-PGDH and the recovery rates were calculated by comparing the measured value to the expected amount of 15-PGDH in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
89-105
97
EDTA plasma(n=5)
85-102
96
heparin plasma(n=5)
88-104
98
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of 15-PGDH and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
89-105%
88-104%
86-102%
EDTA plasma(n=5)
84-100%
82-95%
90-100%
heparin plasma(n=5)
81-100%
80-89%
81-98%
Sensitivity
< 9.375pg/ml
Notes
For Research Use Only
Applications
ELISA
Applications Notes
15-PGDHELISA Kit allows for the in vitro quantitative determination of 15-PGDH concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Mouse 15-PGDH (Prostaglandin dehydrogenase 1) ELISA Kit