Monkey Tumor necrosis factor alpha,TNF-alpha ELISA KIT

Monkey Tumor necrosis factor alpha,TNF-alpha ELISA KIT

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Mfr.No.

CSB-E10039Mo

Sensitivity

0.39 pg/mL

Detection Range

1.56 pg/mL-100 pg/mL

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

tumor necrosis factor (TNF superfamily, member 2), DADB-70P7.1, DIF, TNF-alpha, TNFA, TNFSF2, APC1 protein, TNF superfamily, member 2, TNF, macrophage-derived, TNF, monocyte-derived, cachectin, tumor necrosis factor alpha

Recovery

The recovery of rat NPY spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	93	87-99
EDTA plasma (n=4)	105	100-110

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat NPY in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	95
	Range %	92-104
1:2	Average %	97
	Range %	92-103
1:4	Average %	93
	Range %	89-95
1:8	Average %	86
	Range %	82-92

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
100	2.487	2.385	2.436	2.290
50	1.629	1.527	1.578	1.432
25	0.916	0.937	0.927	0.781
12.5	0.574	0.565	0.570	0.424
6.25	0.375	0.386	0.381	0.235
3.12	0.261	0.250	0.256	0.110
1.56	0.186	0.182	0.184	0.038
0	0.146	0.145	0.146	?

Species

Monkey

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	P48094

Applications	ELISA
Research Area	TNF

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10505020	CSB-E10039Mo	96T	$495	Add to Cart
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BHE10505020	CSB-E10039Mo	10x96T	$4702.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.