Matrices listed below were spiked with certain level of TRACP-5b and the recovery rates were calculated by comparing the measured value to the expected amount of TRACP-5b in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
88-102
93
EDTA plasma(n=5)
85-102
92
heparin plasma(n=5)
94-104
98
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of TRACP-5b and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
85-102%
88-104%
88-103%
EDTA plasma(n=5)
85-100%
91-101%
85-92%
heparin plasma(n=5)
86-100%
87-100%
87-99%
Sensitivity
< 18.75pg/ml
Notes
For Research Use Only
Applications
ELISA
Applications Notes
TRACP-5b ELISA Kit allows for the in vitro quantitative determination of TRACP-5b concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Monkey TRACP-5b(TartRate-resistant Acid Phosphatase 5b) ELISA Kit