Matrices listed below were spiked with certain level of TNFRSF5/CD40 and the recovery rates were calculated by comparing the measured value to the expected amount of TNFRSF5/CD40 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
85-103
96
EDTA plasma(n=5)
89-103
99
heparin plasma(n=5)
86-103
94
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of TNFRSF5/CD40 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
85-105%
85-105%
85-99%
EDTA plasma(n=5)
89-101%
90-98%
87-101%
heparin plasma(n=5)
95-100%
85-96%
87-97%
Sensitivity
< 11.719pg/ml
Notes
For Research Use Only
UniProt ID
A0A7N9D167
Applications
ELISA
Applications Notes
TNFRSF5/CD40 ELISA Kit allows for the in vitro quantitative determination of TNFRSF5/CD40 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Reviews of Monkey TNFRSF5/CD40(Tumor necrosis factor receptor superfamily member 5) ELISA Kit