Matrices listed below were spiked with certain level of TIMP-2 and the recovery rates were calculated by comparing the measured value to the expected amount of TIMP-2 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
95-101
97
EDTA plasma(n=5)
95-103
98
heparin plasma(n=5)
96-103
99
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of TIMP-2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
89-103%
93-105%
91-95%
EDTA plasma(n=5)
90-99%
89-90%
89-94%
heparin plasma(n=5)
90-99%
90-98%
90-99%
Sensitivity
< 0.094ng/ml
Notes
For Research Use Only
Applications
ELISA
Applications Notes
TIMP-2 ELISA Kit allows for the in vitro quantitative determination of TIMP-2 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Monkey TIMP-2(Metalloproteinase inhibitor 2) ELISA Kit