Matrices listed below were spiked with certain level of TIMP-1 and the recovery rates were calculated by comparing the measured value to the expected amount of TIMP-1 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
85-103
93
EDTA plasma(n=5)
88-100
93
heparin plasma(n=5)
86-105
97
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of TIMP-1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
87-99%
89-105%
89-99%
EDTA plasma(n=5)
82-97%
82-101%
83-100%
heparin plasma(n=5)
82-97%
80-91%
86-95%
Sensitivity
< 28.125pg/ml
Notes
For Research Use Only
UniProt ID
A0A0D9RMD6
Applications
ELISA
Applications Notes
TIMP-1 ELISA Kit allows for the in vitro quantitative determination of TIMP-1 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Monkey TIMP-1(Metalloproteinase inhibitor 1) ELISA Kit