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Home / ELISA Kit

Monkey thyroxine (T4) ELISA kit

SKU : BHE10505016

This Human DPD ELISA Kit was designed for the quantitative measurement of Human DPD protein in serum, urine, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 1.56 ng/mL-100 ng/mL and the sensitivity is 0.39 ng/mL.

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CUSABIO TECHONOLOGY LLC

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Mfr.No.

CSB-EQ027512MK

Sensitivity

20 ng/mL

Detection Range

20 ng/mL-320 ng/mL

Assay Duration

1-5h

Assay Type

Competitive

Alternative Names

thyroxine (T4)

Recovery

The recovery of human DPD spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type		Average % Recovery		Range
Serum (n=5)		95		90-100

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human DPD in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?		Sample		Serum(n=4)
1:1		Average %		88
		Range %		80-92
1:2		Average %		94
		Range %		90-100
1:4		Average %		96
		Range %		91-106
1:8		Average %		92
		Range %		80-98

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
100	2.116	2.093	2.105	1.955
50	1.912	1.803	1.858	1.708
25	1.663	1.574	1.619	1.469
12.5	1.258	1.186	1.222	1.072
6.25	0.894	0.867	0.881	0.731
3.12	0.521	0.502	0.512	0.362
1.56	0.314	0.289	0.302	0.152
0	0.152	0.148	0.150	?

Species

Monkey

Limit Of Detection

450 nm

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Protocol

Protocol

Applications	ELISA
Research Area	T4

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price	Quantity
BHE10505016	CSB-EQ027512MK	96T	$495		Add to Cart
BHE10505016	CSB-EQ027512MK	5x96T	$2376		Add to Cart
BHE10505016	CSB-EQ027512MK	10x96T	$4702.50		Add to Cart

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