Matrices listed below were spiked with certain level of RF-IgG and the recovery rates were calculated by comparing the measured value to the expected amount of RF-IgG in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
89-101
94
EDTA plasma(n=5)
90-104
96
heparin plasma(n=5)
89-96
93
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of RF-IgG and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
87-105%
89-99%
89-99%
EDTA plasma(n=5)
90-100%
92-99%
97-101%
heparin plasma(n=5)
94-100%
94-100%
96-99%
Sensitivity
< 1.875IU/ml
Notes
For Research Use Only
Applications
ELISA
Applications Notes
RF-IgG ELISA Kit allows for the in vitro quantitative determination of RF-IgG concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Monkey RF-IgG(Rheumatoid Factor IgG) ELISA Kit