Monkey Proprotein convertase subtilisin/kexin type 9(PCSK9) ELISA kit

Mfr.No.

CSB-EL017647RH

Alternative Names

proprotein convertase subtilisin/kexin type 9, FH3, HCHOLA3, LDLCQ1, NARC-1, NARC1, convertase subtilisin/kexin type 9 preproprotein, neural apoptosis regulated convertase 1

Detection Range

1.1 pg/mL-800 pg/mL

Sample Type

Tissue homogenates, Serum, Plasma

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of rat CNTF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	90	82-95
EDTA plasma (n=4)	108	106-113

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat CNTF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	95
	Range %	92-100
1:2	Average %	90
	Range %	82-95
1:4	Average %	103
	Range %	99-110
1:8	Average %	87
	Range %	82-98

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
1000	2.121	1.985	2.053	1.913
500	1.598	1.543	1.571	1.431
250	1.223	1.144	1.184	1.044
125	0.865	0.911	0.888	0.748
62	0.542	0.513	0.528	0.388
31.2	0.339	0.331	0.335	0.195
15.6	0.257	0.255	0.256	0.116
0	0.142	0.137	0.140	?

Species

Monkey

Sensitivity

1.1 pg/mL

Limit Of Detection

450 nm

UniProt ID	A8T666
Antigen	Proprotein Convertase Subtilisin/Kexin Type 9

Applications

ELISA

Research Area

PCSK9

Protocol

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10505009	CSB-EL017647RH	96T	$790	Add to Cart
BHE10505009	CSB-EL017647RH	5x96T	$3792	Add to Cart
BHE10505009	CSB-EL017647RH	10x96T	$7505	Add to Cart

Monkey Proprotein convertase subtilisin/kexin type 9(PCSK9) ELISA kit

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