Matrices listed below were spiked with certain level of PRL and the recovery rates were calculated by comparing the measured value to the expected amount of PRL in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
89-103
96
EDTA plasma(n=5)
85-104
97
heparin plasma(n=5)
86-104
98
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of PRL and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
86-102%
85-98%
86-101%
EDTA plasma(n=5)
91-98%
89-99%
89-99%
heparin plasma(n=5)
88-100%
90-100%
90-100%
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Notes
For Research Use Only
Applications
ELISA
Applications Notes
PRL ELISA Kit allows for the in vitro quantitative determination of PRL concentrations in serum, plasma, tissue homogenates and other biological fluids.