Monkey primate FGF7/KGF ELISA Kit PicoKine®

Mfr.No.

EK0820-PR

Sensitivity

<4 pg/ml

Detection Range

31.2 pg/ml - 2,000 pg/ml

Reactivity

Monkey

Sample Volume

Concentration (pg/ml)	0	31.2	62.5	125	250	500	1000	2000
O.D.	0.070	0.104	0.129	0.191	0.337	0.635	1.348	1.900

Description

Monkey primate FGF7/KGF ELISA Kit PicoKine® (96 Tests). Quantitate Monkey FGF7 in cell culture supeatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 4pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Fibroblast growth factor 7;FGF-7;Heparin-binding growth factor 7;HBGF-7;Keratinocyte growth factor;FGF7;KGF;

Molecular Weight

22509 MW

Immunogen

Expression system for standard: E.coli; Immunogen sequence: C32-T194

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Subcellular Localization

Secreted.

Sample Type

Cell culture supeatants, Serum, Plasma

Anticoagulant

heparin, EDTA or citrate

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	156	645	1278	147	649	1226
Standard deviation	6.40	27.09	70.29	12.35	39.59	78.46
CV (%)	4.1	4.2	5.6	8.4	6.1	6.4

Protein Function

Plays an important role in the regulation of embryonic development, cell proliferation and cell differentiation. Required for normal branching morphogenesis. Growth factor active on keratinocytes. Possible major paracrine effector of normal epithelial cell proliferation. .

Background

Keratinocyte growth factor is a protein that in humans is encoded by the FGF7 gene. The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This protein is a potent epithelial cell-specific growth factor, whose mitogenic activity is predominantly exhibited in keratinocytes but not in fibroblasts and endothelial cells. Studies of monkey primate and monkey primate homologs of this gene implicated roles in morphogenesis of epithelium, reepithelialization of wounds, hair development and early lung organogenesis.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	574345
Gene Name	FGF7
UniProt ID	P21781
Protein Name	Fibroblast growth factor 7
Tissue Specificity	Epithelial cell.

Applications	ELISA
Research Area	Growth Factors,Growth Factors/Hormones,Organogenesis,Signal Transduction

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21001246	EK0820-PR	96 wells/kit	$499		Add to Cart

Monkey primate FGF7/KGF ELISA Kit PicoKine®

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