Monkey monocyte chemotactic protein 1/monocyte chemotactic and activating factor,MCP1/MCAF ELISA kit

Mfr.No.

CSB-E10045Mo

Sensitivity

15.6 pg/mL

Detection Range

62.5 pg/mL-4000 pg/mL

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

chemokine (C-C motif) ligand 2, GDCF-2, HC11, HSMCR30, MCAF, MCP-1, MCP1, MGC9434, SCYA2, SMC-CF, monocyte chemoattractant protein-1, monocyte chemotactic and activating factor, monocyte secretory protein JE, small inducible cytokine

Recovery

The recovery of rat TNNI1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	88	84-92
EDTA plasma (n=4)	103	99-108

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat TNNI1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	84
	Range %	80-88
1:2	Average %	90
	Range %	86-94
1:4	Average %	95
	Range %	90-100
1:8	Average %	92
	Range %	88-96

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
1000	2.526	2.423	2.475	2.289
500	1.875	1.756	1.816	1.630
250	1.212	1.159	1.186	1.000
125	0.784	0.811	0.798	0.612
62.5	0.500	0.490	0.495	0.309
31.2	0.347	0.362	0.355	0.169
15.6	0.291	0.274	0.283	0.097
0	0.183	0.188	0.186	?

Species

Monkey

Limit Of Detection

450 nm

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Protocol

UniProt ID	P61275

Applications	ELISA
Research Area	CCL2

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10505003	CSB-E10045Mo	96T	$595	Add to Cart
BHE10505003	CSB-E10045Mo	5x96T	$2856	Add to Cart
BHE10505003	CSB-E10045Mo	10x96T	$5652.50	Add to Cart

Monkey monocyte chemotactic protein 1/monocyte chemotactic and activating factor,MCP1/MCAF ELISA kit

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