Monkey Leptin,LEP ELISA Kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-E14936Mk

Sensitivity

3.125 ng/mL

Detection Range

3.125 ng/mL- 50 ng/mL

Assay Duration

1-5h

Assay Type

Competitive

Alternative Names

leptin, FLJ94114, OB, OBS, leptin (murine obesity homolog), leptin (obesity homolog, mouse), obese, mouse, homolog of, obesity factor

Recovery

The recovery of human peptide YY spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	91	87-97
EDTA plasma (n=4)	99	93-105

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human peptide YY in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	91
	Range %	87-97
1:2	Average %	99
	Range %	96-104
1:4	Average %	101
	Range %	95-106
1:8	Average %	95
	Range %	90-98

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Monkey

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	Q28504

Applications	ELISA
Research Area	LEP

Storage	Store at 2-8°C. Please refer to protocol.

Reference	Glucocorticoid treatment facilitates development of a metabolic syndrome in ovariectomized Macaca Mulatta fed a high fat diet. Li L.et al,Steroids,Oct 4

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