Monkey Interferon alpha,IFN-alpha ELISA Kit

Mfr.No.

CSB-E10040Mo

Alternative Names

Interferon alpha,IFN-alpha, IFN-alpha,interferon alpha

Detection Range

15.6 pg/mL-1000 pg/mL

Sample Type

Tissue homogenates, Serum, Urine, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of human PTRF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	83	80-86
EDTA plasma (n=4)	101	98-104

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human PTRF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	89
	Range %	85-92
1:2	Average %	107
	Range %	103-110
1:4	Average %	85
	Range %	80-90
1:8	Average %	91
	Range %	87-96

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Monkey

Sensitivity

3.9 pg/mL

Limit Of Detection

450 nm

Antigen	Interferon α,IFN-α

Applications

ELISA

Research Area

IFNA1

Protocol

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
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