Matrices listed below were spiked with certain level of IL-6 and the recovery rates were calculated by comparing the measured value to the expected amount of IL-6 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
85-98
92
EDTA plasma(n=5)
87-105
94
heparin plasma(n=5)
90-105
95
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IL-6 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
86-104%
87-96%
85-105%
EDTA plasma(n=5)
85-97%
86-97%
89-100%
heparin plasma(n=5)
86-99%
87-99%
86-98%
Sensitivity
< 0.188pg/ml
Notes
For Research Use Only
UniProt ID
P79341
Applications
ELISA
Applications Notes
IL-6 ELISA Kit allows for the in vitro quantitative determination of IL-6 concentrations in serum, plasma, tissue homogenates and other biological fluids.