Matrices listed below were spiked with certain level of IL-22 and the recovery rates were calculated by comparing the measured value to the expected amount of IL-22 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
90-103
96
EDTA plasma(n=5)
88-103
97
heparin plasma(n=5)
86-100
92
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IL-22 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
85-100%
87-105%
86-102%
EDTA plasma(n=5)
89-99%
87-101%
94-100%
heparin plasma(n=5)
86-99%
90-97%
85-97%
Sensitivity
< 1.875pg/ml
Notes
For Research Use Only
Applications
ELISA
Applications Notes
IL-22 ELISA Kit allows for the in vitro quantitative determination of IL-22 concentrations in serum, plasma, tissue homogenates and other biological fluids.