Matrices listed below were spiked with certain level of IL-17 and the recovery rates were calculated by comparing the measured value to the expected amount of IL-17 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
89-105
98
EDTA plasma(n=5)
91-102
96
heparin plasma(n=5)
89-100
94
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IL-17 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
90-104%
92-105%
90-102%
EDTA plasma(n=5)
94-101%
89-97%
92-100%
heparin plasma(n=5)
95-100%
96-100%
95-100%
Sensitivity
< 9.375pg/ml
Notes
For Research Use Only
UniProt ID
A0A2K5TTB0
Applications
ELISA
Applications Notes
IL-17 ELISA Kit allows for the in vitro quantitative determination of IL-17 concentrations in serum, plasma, tissue homogenates and other biological fluids.