Matrices listed below were spiked with certain level of IGFBP-3 and the recovery rates were calculated by comparing the measured value to the expected amount of IGFBP-3 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
85-102
91
EDTA plasma(n=5)
88-98
91
heparin plasma(n=5)
86-98
93
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IGFBP-3 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
91-104%
96-104%
86-104%
EDTA plasma(n=5)
85-92%
85-101%
86-97%
heparin plasma(n=5)
89-99%
85-90%
85-99%
Sensitivity
< 0.094ng/ml
Notes
For Research Use Only
UniProt ID
A0A7N9C7B5
Applications
ELISA
Applications Notes
IGFBP-3 ELISA Kit allows for the in vitro quantitative determination of IGFBP-3 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Monkey IGFBP-3(Insulin-like growth factor-binding protein 3) ELISA Kit