Matrices listed below were spiked with certain level of IDO1 and the recovery rates were calculated by comparing the measured value to the expected amount of IDO1 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
87-103
94
EDTA plasma(n=5)
91-99
93
heparin plasma(n=5)
86-98
90
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IDO1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
92-105%
89-105%
85-101%
EDTA plasma(n=5)
87-101%
85-100%
86-101%
heparin plasma(n=5)
85-94%
85-94%
85-95%
Sensitivity
< 0.094ng/ml
Notes
For Research Use Only
UniProt ID
A0A2K5TU29
Applications
ELISA
Applications Notes
IDO1 ELISA Kit allows for the in vitro quantitative determination of IDO1 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Reviews of Monkey IDO1(Indoleamine 2,3-dioxygenase 1) ELISA Kit