Matrices listed below were spiked with certain level of GPT and the recovery rates were calculated by comparing the measured value to the expected amount of GPT in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
93-104
97
EDTA plasma(n=5)
89-105
99
heparin plasma(n=5)
90-96
93
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of GPT and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
88-100%
88-100%
93-105%
EDTA plasma(n=5)
91-96%
90-98%
90-100%
heparin plasma(n=5)
94-98%
95-97%
94-97%
Sensitivity
< 0.094ng/ml
Notes
For Research Use Only
UniProt ID
A0A2K5VX98
Applications
ELISA
Applications Notes
GPT ELISA Kit allows for the in vitro quantitative determination of GPT concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Monkey GPT(Alanine aminotransferase 1) ELISA Kit