Matrices listed below were spiked with certain level of GLP-1,active form and the recovery rates were calculated by comparing the measured value to the expected amount of GLP-1,active form in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
88-102
97
EDTA plasma(n=5)
85-97
92
heparin plasma(n=5)
85-89
87
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of GLP-1,active form and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
85-104%
88-102%
85-101%
EDTA plasma(n=5)
91-101%
85-99%
86-96%
heparin plasma(n=5)
85-100%
85-98%
85-98%
Sensitivity
< 9.375ng/ml
Notes
For Research Use Only
Applications
ELISA
Applications Notes
GLP-1,active form ELISA Kit allows for the in vitro quantitative determination of GLP-1,active form concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Monkey GLP-1,active form (Glucagon Like Peptide 1,active form) ELISA Kit