Matrices listed below were spiked with certain level of D2D and the recovery rates were calculated by comparing the measured value to the expected amount of D2D in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
85-98
91
EDTA plasma(n=5)
89-103
98
heparin plasma(n=5)
85-98
91
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of D2D and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
93-105%
85-102%
91-104%
EDTA plasma(n=5)
82-99%
87-101%
82-100%
heparin plasma(n=5)
81-96%
80-92%
82-98%
Sensitivity
< 46.875pg/ml
Notes
For Research Use Only
Applications
ELISA
Applications Notes
D2D ELISA Kit allows for the in vitro quantitative determination of D2D concentrations in serum, plasma, tissue homogenates and other biological fluids.