Monkey complement C5 (C5) ELISA kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-EL003995MK

Sensitivity

0.156 ng/mL

Detection Range

0.625 ng/mL-40 ng/mL

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

complement C5a anaphylatoxin, CPAMD4, FLJ17816, FLJ17822, MGC142298, anaphylatoxin C5a analog

Recovery

The recovery of rat C3a spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	90-97
EDTA plasma (n=4)	101	95-107

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat C3a in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:5	Average %	88
	Range %	80-97
1:10	Average %	95
	Range %	92-102
1:20	Average %	96
	Range %	90-102
1:40	Average %	93
	Range %	90-96

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Monkey

Limit Of Detection

450 nm

Precision

Protocol

Applications	ELISA
Research Area	C5

Storage	Store at 2-8°C. Please refer to protocol.

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