Matrices listed below were spiked with certain level of CD30L and the recovery rates were calculated by comparing the measured value to the expected amount of CD30L in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
86-102
92
EDTA plasma(n=5)
91-105
99
heparin plasma(n=5)
87-102
96
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of CD30L and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
88-97%
93-103%
86-100%
EDTA plasma(n=5)
86-101%
86-99%
86-101%
heparin plasma(n=5)
88-98%
85-97%
88-96%
Sensitivity
< 4.688pg/ml
Notes
For Research Use Only
UniProt ID
G7P2F1
Applications
ELISA
Applications Notes
CD30L ELISA Kit allows for the in vitro quantitative determination of CD30L concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Reviews of Monkey CD30L(Tumor necrosis factor ligand superfamily member 8) ELISA Kit