Matrices listed below were spiked with certain level of CA19-9 and the recovery rates were calculated by comparing the measured value to the expected amount of CA19-9 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
83-105
93
EDTA plasma(n=5)
87-100
95
heparin plasma(n=5)
86-101
97
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of CA19-9 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
83-95%
88-104%
88-105%
EDTA plasma(n=5)
91-101%
92-101%
90-101%
heparin plasma(n=5)
93-100%
90-97%
91-100%
Sensitivity
< 1.875IU/ml
Notes
For Research Use Only
Applications
ELISA
Applications Notes
CA19-9 ELISA Kit allows for the in vitro quantitative determination of CA19-9 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Monkey CA19-9(Carbohydrate antigen19-9) ELISA Kit