Matrices listed below were spiked with certain level of C5b and the recovery rates were calculated by comparing the measured value to the expected amount of C5b in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
87-103
95
EDTA plasma(n=5)
85-104
92
heparin plasma(n=5)
91-102
96
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of C5b and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
86-96%
85-100%
85-105%
EDTA plasma(n=5)
87-100%
86-100%
91-101%
heparin plasma(n=5)
89-95%
87-100%
87-100%
Sensitivity
< 18.75pg/ml
Notes
For Research Use Only
UniProt ID
A0A2K5U1T1
Applications
ELISA
Applications Notes
C5b ELISA Kit allows for the in vitro quantitative determination of C5b concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Monkey C5b(Complement Component 5b) ELISA Kit