Matrices listed below were spiked with certain level of C5b-9 and the recovery rates were calculated by comparing the measured value to the expected amount of C5b-9 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
89-102
97
EDTA plasma(n=5)
90-103
97
heparin plasma(n=5)
92-104
99
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of C5b-9 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
85-96%
85-103%
97-104%
EDTA plasma(n=5)
91-100%
92-101%
93-100%
heparin plasma(n=5)
93-99%
90-96%
91-99%
Sensitivity
< 0.188ng/ml
Notes
For Research Use Only
Applications
ELISA
Applications Notes
C5b-9 ELISA Kit allows for the in vitro quantitative determination of C5b-9 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Monkey C5b-9 (Terminal Complement Complex C5b-9) ELISA Kit