Matrices listed below were spiked with certain level of C-P and the recovery rates were calculated by comparing the measured value to the expected amount of C-P in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
85-105
96
EDTA plasma(n=5)
90-102
96
heparin plasma(n=5)
89-103
96
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of C-P and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
94-102%
90-99%
85-102%
EDTA plasma(n=5)
84-95%
88-98%
96-99%
heparin plasma(n=5)
83-100%
84-95%
89-100%
Sensitivity
< 18.75pg/ml
Notes
For Research Use Only
UniProt ID
P30406
Applications
ELISA
Applications Notes
C-P ELISA Kit allows for the in vitro quantitative determination of C-P concentrations in serum, plasma, tissue homogenates and other biological fluids.