Matrices listed below were spiked with certain level of beta-CTx and the recovery rates were calculated by comparing the measured value to the expected amount of beta-CTx in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
88-104
93
EDTA plasma(n=5)
89-105
96
heparin plasma(n=5)
94-105
99
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of beta-CTx and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
93-102%
90-102%
86-105%
EDTA plasma(n=5)
93-98%
87-96%
85-90%
heparin plasma(n=5)
90-99%
87-99%
85-100%
Sensitivity
< 1.875pg/ml
Notes
For Research Use Only
Applications
ELISA
Applications Notes
beta-CTx ELISA Kit allows for the in vitro quantitative determination of beta-CTx concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Monkey beta-CTx(Beta Crosslaps) ELISA Kit