Matrices listed below were spiked with certain level of ANP and the recovery rates were calculated by comparing the measured value to the expected amount of ANP in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
88-103
97
EDTA plasma(n=5)
85-103
93
heparin plasma(n=5)
89-104
95
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ANP and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
86-105%
86-104%
89-98%
EDTA plasma(n=5)
84-98%
88-100%
83-97%
heparin plasma(n=5)
86-93%
83-98%
81-93%
Sensitivity
< 9.375pg/ml
Notes
For Research Use Only
Applications
ELISA
Applications Notes
ANPELISA Kit allows for the in vitro quantitative determination of ANP concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Monkey ANP(Atrial Natriuretic Peptide)ELISA Kit