Matrices listed below were spiked with certain level of alpha1-AT and the recovery rates were calculated by comparing the measured value to the expected amount of alpha1-AT in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
92-103
98
EDTA plasma(n=5)
85-97
90
heparin plasma(n=5)
88-101
95
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of alpha1-AT and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
86-93%
92-105%
88-99%
EDTA plasma(n=5)
85-101%
87-97%
91-98%
heparin plasma(n=5)
85-100%
88-97%
85-98%
Sensitivity
< 0.094ng/ml
Notes
For Research Use Only
UniProt ID
G7PBD8
Applications
ELISA
Applications Notes
alpha1-AT ELISA Kit allows for the in vitro quantitative determination of alpha1-AT concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Monkey alpha1-AT(Alpha 1-Antitrypsin) ELISA Kit