Matrices listed below were spiked with certain level of AGT and the recovery rates were calculated by comparing the measured value to the expected amount of AGT in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
88-101
94
EDTA plasma(n=5)
86-104
97
heparin plasma(n=5)
85-104
93
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of AGT and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
88-98%
86-102%
86-103%
EDTA plasma(n=5)
84-100%
85-100%
86-100%
heparin plasma(n=5)
80-96%
81-97%
98-100%
Sensitivity
< 0.469ng/ml
Notes
For Research Use Only
UniProt ID
A0A0D9RDV1
Applications
ELISA
Applications Notes
AGTELISA Kit allows for the in vitro quantitative determination of AGT concentrations in serum, plasma, tissue homogenates and other biological fluids.