Mouse Procollagen I N-Terminal Propeptide (PINP) (Competitive EIA) ELISA Kit

Mfr.No.

MEA957Mu

Sensitivity

1.44ng/mL

Detection Range

3.70-300ng/mL

Reactivity

Mouse

Assay Duration

Assay Type

Competitive Inhibition

Description

This assay has high sensitivity and excellent specificity for detection of Mini Samples Procollagen I N-Terminal Propeptide (PINP).
No significant cross-reactivity or interference between Mini Samples Procollagen I N-Terminal Propeptide (PINP) and analogues was observed.

Alternative Names

P1NP; N-Propeptide Of Type I Procollagen; Procollagen I Amino Terminal Propeptide

Detection Method

Colormetric

Kit Components

Reagents	Quantity	Reagents	Quantity
Pre-coated, ready to use 96-well strip plate	1	Plate sealer for 96 wells	4
Standard	2	Standard Diluent	1×20mL
Detection Reagent A	1×60µL	Assay Diluent A	1×6mL
Detection Reagent B	1×60µL	Assay Diluent B	1×6mL
TMB Substrate	1×9mL	Stop Solution	1×3mL
Wash Buffer (30 × concentrate)	1×10mL	Instruction manual	1

Recovery

Matrices listed below were spiked with certain level of recombinant Mini Samples Procollagen I N-Terminal Propeptide (PINP) and the recovery rates were calculated by comparing the measured value to the expected amount of Mini Samples Procollagen I N-Terminal Propeptide (PINP) in samples.

Matrix	Recovery range (%)	Average(%)
serum(n=5)	96-104	101
EDTA plasma(n=5)	79-90	82
heparin plasma(n=5)	80-102	84

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Mini Samples Procollagen I N-Terminal Propeptide (PINP) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1:2	1:4	1:8	1:16
serum(n=5)	95-103%	93-101%	86-101%	92-99%
EDTA plasma(n=5)	83-102%	96-105%	83-101%	85-99%
heparin plasma(n=5)	89-103%	78-102%	93-101%	86-95%

Features

Detects protein (micro-volume samples 15-25 ?l)

Species

Mouse

Precautions

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Mini Samples Procollagen I N-Terminal Propeptide (PINP) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Mini Samples Procollagen I N-Terminal Propeptide (PINP) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: C<10%
Inter-Assay: C<12%

Notes

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Mini Samples Procollagen I N-Terminal Propeptide (PINP) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Mini Samples Procollagen I N-Terminal Propeptide (PINP) and unlabeled Mini Samples Procollagen I N-Terminal Propeptide (PINP) (Standards or samples) with the pre-coated antibody specific to Mini Samples Procollagen I N-Terminal Propeptide (PINP). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Mini Samples Procollagen I N-Terminal Propeptide (PINP) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Mini Samples Procollagen I N-Terminal Propeptide (PINP) in the sample.

Usage Notes

1. Prepare all reagents, samples and standards;
2. Add 25µL standard or sample to each well.
And then add 25?L prepared Detection Reagent A immediately.
Shake and mix. Incubate 1 hour at 37°C;
3. Aspirate and wash 3 times;
4. Add 50µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
5. Aspirate and wash 5 times;
6. Add 50µL Substrate Solution. Incubate 10-20 minutes at 37°C;
7. Add 25µL Stop Solution. Read at 450 nm immediately.

Gene ID	12842
UniProt ID	P11087

Applications	ELISA
Research Area	Metabolic pathway

Storage	4°C for 1 month/ -20°C for long-term(within shelf life)
Shelf Life	one year

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SKU	Supplier No.	Size	Your price
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Mini Samples ELISA Kit for Procollagen I N-Terminal Propeptide (PINP) (Mouse)

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