General species Indole 3 Acetic Acid (IAA) (Competitive EIA) ELISA Kit

Mfr.No.

MEA737Ge

Sensitivity

112pg/mL

Detection Range

320-200,000pg/mL

Reactivity

General

Assay Duration

Assay Type

Competitive Inhibition

Description

This assay has high sensitivity and excellent specificity for detection of Mini Samples Indole 3 Acetic Acid (IAA).
No significant cross-reactivity or interference between Mini Samples Indole 3 Acetic Acid (IAA) and analogues was observed.

Alternative Names

IA-A; Indolylacetic Acid; Indoleacetic Acid; Heteroauxin

Detection Method

Colormetric

Kit Components

Reagents	Quantity	Reagents	Quantity
Pre-coated, ready to use 96-well strip plate	1	Plate sealer for 96 wells	4
Standard	2	Standard Diluent	1×20mL
Detection Reagent A	1×60µL	Assay Diluent A	1×6mL
Detection Reagent B	1×60µL	Assay Diluent B	1×6mL
TMB Substrate	1×9mL	Stop Solution	1×3mL
Wash Buffer (30 × concentrate)	1×10mL	Instruction manual	1

Features

Detects protein (micro-volume samples 15-25 ?l)

Species

General species

Precautions

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Mini Samples Indole 3 Acetic Acid (IAA) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Mini Samples Indole 3 Acetic Acid (IAA) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: C<10%
Inter-Assay: C<12%

Notes

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Mini Samples Indole 3 Acetic Acid (IAA) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Mini Samples Indole 3 Acetic Acid (IAA) and unlabeled Mini Samples Indole 3 Acetic Acid (IAA) (Standards or samples) with the pre-coated antibody specific to Mini Samples Indole 3 Acetic Acid (IAA). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Mini Samples Indole 3 Acetic Acid (IAA) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Mini Samples Indole 3 Acetic Acid (IAA) in the sample.

Usage Notes

1. Prepare all reagents, samples and standards;
2. Add 25µL standard or sample to each well.
And then add 25?L prepared Detection Reagent A immediately.
Shake and mix. Incubate 1 hour at 37°C;
3. Aspirate and wash 3 times;
4. Add 50µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
5. Aspirate and wash 5 times;
6. Add 50µL Substrate Solution. Incubate 10-20 minutes at 37°C;
7. Add 25µL Stop Solution. Read at 450 nm immediately.

Applications	ELISA
Research Area	Signal transduction

Storage	4°C for 1 month/ -20°C for long-term(within shelf life)
Shelf Life	one year

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SKU	Supplier No.	Size	Your price
BHE10109262	MEA737Ge	96T*1	$955	Add to Cart
BHE10109262	MEA737Ge	96T*5	$4297.50	Add to Cart
BHE10109262	MEA737Ge	96T*10	$8117.50	Add to Cart

Mini Samples ELISA Kit for Indole 3 Acetic Acid (IAA) (General species)

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