Human Galectin 1 (GAL1) (Sandwich ELISA) ELISA Kit

Mfr.No.

MEA321Hu

Sensitivity

0.115ng/mL

Detection Range

0.312-20ng/mL

Reactivity

Human

Assay Duration

Assay Type

Sandwich

Sample Volume

15-25 ul(Please refer to the manual for the specific data.)

Description

This assay has high sensitivity and excellent specificity for detection of Mini Samples Galectin 1 (GAL1).
No significant cross-reactivity or interference between Mini Samples Galectin 1 (GAL1) and analogues was observed.

Alternative Names

LGALS1; GBP; HLBP14; Galaptin; Lectin,Galactoside-Binding Soluble 1; 14 kDa laminin-binding protein; Lactose-binding lectin 1; Putative MAPK-activating protein PM12; S-Lac lectin

Detection Method

Colormetric

Kit Components

Reagents	Quantity	Reagents	Quantity
Pre-coated, ready to use 96-well strip plate	1	Plate sealer for 96 wells	4
Standard	2	Standard Diluent	1×20mL
Detection Reagent A	1×60µL	Assay Diluent A	1×6mL
Detection Reagent B	1×60µL	Assay Diluent B	1×6mL
TMB Substrate	1×4.5mL	Stop Solution	1×3mL
Wash Buffer (30 × concentrate)	1×10mL	Instruction manual	1

Recovery

Matrices listed below were spiked with certain level of recombinant Mini Samples Galectin 1 (GAL1) and the recovery rates were calculated by comparing the measured value to the expected amount of Mini Samples Galectin 1 (GAL1) in samples.

Matrix	Recovery range (%)	Average(%)
serum(n=5)	86-101	94
EDTA plasma(n=5)	85-94	90
heparin plasma(n=5)	98-105	101

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Mini Samples Galectin 1 (GAL1) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1:2	1:4	1:8	1:16
serum(n=5)	98-105%	96-104%	97-104%	91-101%
EDTA plasma(n=5)	80-91%	88-96%	78-97%	79-104%
heparin plasma(n=5)	79-101%	93-101%	99-105%	86-98%

Features

Detects protein (micro-volume samples 15-25 ?l)

Species

Human

Sample Type

Cell lysates, Cell culture supeates, Serum, Tissue homogenates, Plasma, Other biological fluids

Precautions

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Mini Samples Galectin 1 (GAL1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Mini Samples Galectin 1 (GAL1) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: C<10%
Inter-Assay: C<12%

Notes

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mini Samples Galectin 1 (GAL1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Mini Samples Galectin 1 (GAL1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mini Samples Galectin 1 (GAL1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mini Samples Galectin 1 (GAL1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Usage Notes

1. Prepare all reagents, samples and standards;
2. Add 25µL standard or sample to each well. Incubate 1 hour at 37°C;
3. Aspirate and add 25µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 25µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 25µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 20µL Stop Solution. Read at 450nm immediately.

Gene ID	3956
UniProt ID	P09382
Tissue Specificity	Reactive with Human GAL1 / Galectin 1

Applications	ELISA
Research Area	Tumor immunity

Storage	4°C for 1 month/ -20°C for long-term(within shelf life)
Shelf Life	one year

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Mini Samples ELISA Kit for Galectin 1 (GAL1) (Human)

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