Matrices listed below were spiked with certain level of MDA and the recovery rates were calculated by comparing the measured value to the expected amount of MDA in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
86-101
92
EDTA plasma(n=5)
85-105
95
heparin plasma(n=5)
85-103
98
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of MDA and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
MDA ELISA Kit allows for the in vitro quantitative determination of MDA concentrations in serum, plasma, tissue homogenates and other biological fluids.