Rat Procollagen I N-Terminal Propeptide (PINP) (Sandwich ELISA) ELISA Kit

Mfr.No.

IEA957Ra

Sensitivity

92.4pg/mL

Detection Range

246.9-20,000pg/mL

Reactivity

Rat

Assay Duration

1h, 10min

Assay Type

Sandwich

Description

This assay has high sensitivity and excellent specificity for detection of Instant Procollagen I N-Terminal Propeptide (PINP).
No significant cross-reactivity or interference between Instant Procollagen I N-Terminal Propeptide (PINP) and analogues was observed.

Alternative Names

P1NP; N-Propeptide Of Type I Procollagen; Procollagen I Amino Terminal Propeptide

Detection Method

Colormetric

Kit Components

Reagents	Quantity	Reagents	Quantity
Pre-coated, ready to use 96-well strip plate	1	Plate sealer for 96 wells	4
Standard	2	Standard Diluent	1×20mL
Detection Reagent A	1×120µL	Assay Diluent A	1×12mL
Detection Reagent B	1×120µL	Assay Diluent B	1×12mL
TMB Substrate	1×9mL	Stop Solution	1×6mL
Wash Buffer (30 × concentrate)	1×20mL	Instruction manual	1

Recovery

Matrices listed below were spiked with certain level of recombinant Instant Procollagen I N-Terminal Propeptide (PINP) and the recovery rates were calculated by comparing the measured value to the expected amount of Instant Procollagen I N-Terminal Propeptide (PINP) in samples.

Matrix	Recovery range (%)	Average(%)
serum(n=5)	83-103	98
EDTA plasma(n=5)	93-101	96
heparin plasma(n=5)	90-98	94

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Instant Procollagen I N-Terminal Propeptide (PINP) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1:2	1:4	1:8	1:16
serum(n=5)	88-95%	78-88%	94-102%	92-101%
EDTA plasma(n=5)	93-102%	85-92%	78-91%	95-103%
heparin plasma(n=5)	82-102%	80-93%	85-95%	99-105%

Features

Detects protein (quick procedure in 1 hrs)

Species

Rat

Precautions

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Instant Procollagen I N-Terminal Propeptide (PINP) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Instant Procollagen I N-Terminal Propeptide (PINP) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: C<10%
Inter-Assay: C<12%

Notes

The test principle applied in this kit is enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Instant Procollagen I N-Terminal Propeptide (PINP). Standards or samples and HRP-labeled detection antibody specific to Instant Procollagen I N-Terminal Propeptide (PINP) (Detection Reagent A) are then added to the appropriate microtiter plate wells. Next, TMB substrate solution is added, only those wells that contain Instant Procollagen I N-Terminal Propeptide (PINP), and HRP-labeled detection antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Instant Procollagen I N-Terminal Propeptide (PINP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Usage Notes

1. Prepare all reagents, samples and standards;
2. Add 50µL standard or sample to each well.
And then add 50µL prepared Detection Reagent A immediately.
Share and mix. Incubate 50 minutes at 37°C;
3. Aspirate and wash 3 times;
4. Add 100µL prepared Detection Reagent B. Incubate 10 minutes at 37°C;
5. Aspirate and wash 5 times;
6. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
7. Add 50µL Stop Solution. Read at 450nm immediately.

Gene ID	29393
UniProt ID	P02454

Applications	ELISA
Research Area	Metabolic pathway

Storage	4°C for 1 month/ -20°C for long-term(within shelf life)
Shelf Life	one year

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SKU	Supplier No.	Size	Your price
BHE10103206	IEA957Ra	96T*1	$1026	Add to Cart
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Instant ELISA Kit for Procollagen I N-Terminal Propeptide (PINP) (Rat)

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