Human Zona Pellucida Sperm-binding Protein 3

Mfr.No.

E6422Hu

Sensitivity

9.73ng/L

Detection Range

20-4500ng/L

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

OOMD3;Processed zona pellucida sperm-binding protein 3);Sperm receptor;Zona pellucida glycoprotein 3;Zona pellucida protein C;Zona pellucida sperm-binding protein 3;ZP3;Zp-3;ZP3A;ZP3A/ZP3B;ZP3B;ZPC

Molecular Weight

47018

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E6422Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	492.23	21.95	4.46
Intra-Assay	2	18	1394.65	38.58	2.77
Intra-Assay	3	18	1053.45	39.59	3.76

Background

Component of the zona pellucida, an extracellular matrix surrounding oocytes which mediates sperm binding, induction of the acrosome reaction and prevents post-fertilization polyspermy. The zona pellucida is composed of 3 to 4 glycoproteins, ZP1, ZP2, ZP3, and ZP4. ZP3 is essential for sperm binding and zona matrix formation.

Protocol

These standard curves of E6422Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
2400ng/L	2.093	2.009	1.964
	1.925
1200ng/L	1.06	1.09	1.046
	1.121
600ng/L	0.524	0.556	0.512
	0.589
300ng/L	0.187	0.204	0.16
	0.221
150ng/L	0.14	0.133	0.089
	0.125
0ng/L	0.06	0.044	0
	0.029

Gene ID	7784
UniProt ID	P21754

Research Area	Tags & Cell Markers

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12107784	E6422Hu	48T	$320		Add to Cart
BHE12107784	E6422Hu	96T	$458		Add to Cart

Human Zona Pellucida Sperm-binding Protein 3 - ZP3 ELISA Kit

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