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Home / ELISA Kit

Human Zinc transporter 8 (SLC30A8) ELISA Kit

SKU : BHE13702821

SLC30A8 is expressed at a high level only in the pancreas, particularly in islets of Langerhans. The protein colocalizes with insulin in the secretory pathway granules of the insulin-secreting INS-1 cells. Allelic variants of this gene exist that confer susceptibility to diabetes mellitus, noninsulin-dependent. Several transcript variants encoding different isoforms have been found for this gene.It was also expressed in fetal pancreas, islets of Langerhans, and an insulinoma cell line, but not in HeLa cells. Fluorescence-tagged ZNT8 was expressed in a punctate pattern in the cytoplasm of transfected insulinoma cells and HeLa cells. In insulinoma cells, ZNT8 expression overlapped that of insulin, suggesting that ZNT8 is associated with insulin granule secretion.

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Mfr.No.	KTE60605
Description	This Human Zinc transporter 8 (SLC30A8) ELISA Kit employs a two-site sandwich ELISA to quantitate SLC30A8.
Alternative Names	SLC30A8; ZNT8; ZnT-8; solute carrier family 30 member 8; zinc transporter ZnT-8
Reactivity	Human
Detection Method	Colorimetric
Sample Type	Other biological fluids, Cell culture supernatants, Serum, Plasma
Assay Type	Sandwich (quantitative)
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Kit Components	Human Zinc transporter 8 microplate Human Zinc transporter 8 standard Human Zinc transporter 8 detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Zinc transporter 8 (SLC30A8) ELISA Kit has high sensitivity and excellent specificity for detection of Human SLC30A8. No significant cross-reactivity or interference between Human SLC30A8 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	SLC30A8 is expressed at a high level only in the pancreas, particularly in islets of Langerhans. The protein colocalizes with insulin in the secretory pathway granules of the insulin-secreting INS-1 cells. Allelic variants of this gene exist that confer susceptibility to diabetes mellitus, noninsulin-dependent. Several transcript variants encoding different isoforms have been found for this gene.It was also expressed in fetal pancreas, islets of Langerhans, and an insulinoma cell line, but not in HeLa cells. Fluorescence-tagged ZNT8 was expressed in a punctate pattern in the cytoplasm of transfected insulinoma cells and HeLa cells. In insulinoma cells, ZNT8 expression overlapped that of insulin, suggesting that ZNT8 is associated with insulin granule secretion.
Notes	This Human Zinc transporter 8 (SLC30A8) ELISA Kit employs a two-site sandwich ELISA to quantitate SLC30A8 in samples. An antibody specific for SLC30A8 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySLC30A8 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SLC30A8 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SLC30A8 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Gene ID	169026
Antigen	SLC30A8
Tag	SLC30A8
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Applications	ELISA
Applications Notes	This Human Zinc transporter 8 (SLC30A8) ELISA Kit employs a two-site sandwich ELISA to quantitate SLC30A8 in samples. An antibody specific for SLC30A8 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySLC30A8 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SLC30A8 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SLC30A8 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13702821	KTE60605	48 T	$339		Add to Cart
BHE13702821	KTE60605	96 T	$589		Add to Cart
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BHE13702821	KTE60605	96 Tx50	$24569		Add to Cart

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